Glut4 Antibody (925932) [Alexa Fluor® 488]

HEK293 human embryonic kidney cell line transfected with either human Glut4 (filled histogram) or irrelevant protein (open histogram) were stained with Mouse Anti-Human Glut4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB86541G). Cell were gated based on control antibody staining (IC0041G, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 925932
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Glut4 Antibody (925932) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glut4
  Met1-Asp509
  Accession # P14672
• Specificity: Detects human Glut4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Reconstitution Instructions: Reconstitution default not known

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut4 Antibody (925932) [Alexa Fluor® 488]

• Glut4
• GLUT-4
• GLUT4Glucose transporter type 4, insulin-responsive
• insulin-responsive glucose transporter type 4
• SLC2A4
• solute carrier family 2 (facilitated glucose transporter), member 4
• solute carrier family 2, facilitated glucose transporter member 4

Background

Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein and shares 65% aa identity with mouse Glut4.  It is an insulin-regulated glucose transporter.  Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer.  Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients.  Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut4 Antibody (FAB86541G)(1)

Publication using FAB86541G

• Clemen, R;Dethloff, W;Berner, J;Schulan, P;Martinet, A;Weltmann, KD;von Woedtke, T;Grune, T;Wende, K;Bekeschus, S; Insulin oxidation and oxidative modifications alter glucose uptake, cell metabolism, and inflammatory secretion profiles Redox biology 2024-10-05 [PMID: 39378614] (Immunocytochemistry, Human)

Bioinformatics

• Uniprot:
  • P14672()