Human GLP1 ELISA Kit (Colorimetric)

ELISA: Human GLP1 ELISA Kit (Colorimetric) [NBP3-12819] - Samples were spiked with high concentrations of Human GLP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human GLP1 ELISA Kit (Colorimetric) [NBP3-12819] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 1.56 - 100 pg/mL.
• Sensitivity: 0.94 pg/mL

Human GLP1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human GLP1 in samples. No significant cross reactivity or interference between Human GLP1 and analogues was observed
• Standard Curve Range: 1.56 - 100 pg/mL.
• Sensitivity: 0.94 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.44%
• Intra-Assay: CV% < 5.45%
• Spike Recovery: average = 101% for serum; average = 103% for EDTA plasma; average = 101% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GCG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human GLP1 ELISA Kit (Colorimetric)

• glicentin-related polypeptide
• GLP1
• GLP-1
• glucagon-like peptide 1
• GRPP

Background

Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from enteroendocrine L cells in response to ingested nutrients. The closely related peptides glucagon-like peptide (GLP-1) and glucagon have opposing effects on blood glucose. GLP-1 induces glucose-dependent insulin secretion in the pancreas, while glucagon stimulates gluconeogenesis and glycogenolysis in the liver. Glucagon is processed from a large precursor, proglucagon, in a tissue-specific manner in pancreatic alpha-cells. The identification of a hybrid peptide acting as both a GLP-1 agonist and a glucagon antagonist would provide a novel approach for the treatment of type 2 diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for GLP1 ELISA Kit (NBP3-12819). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GCG