GLP1 Antibody (970307) [Unconjugated]

GLP-1/Glucagon was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GLP-1 Monoclonal Antibody (Catalog # MAB12491) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 970307
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GLP1 Antibody (970307) [Unconjugated] Summary

• Immunogen: Human GLP-1 synthetic peptide
  Accession # P01275
• Specificity: Detects human GLP-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLP1 Antibody (970307) [Unconjugated]

• glicentin-related polypeptide
• GLP1
• GLP-1
• glucagon-like peptide 1
• GRPP

Background

Glucagon is a preprotein which is cleaved into four distinct peptides, including the hormone GLP-1 (aa 98-128).  GLP-1 is a secreted hormone with multiple effects upon the intestine (gastric motility), pancreas (glucose dependent insulin release) and hypothalamic pituitary axis (modulates LH, THS, CRH, ocytocin and vasopressin secretion). It also affects plasma glucagon levels.  GLP-1 is expressed in enteroendocrine L cells and neurons of the caudal brainstem which project to the forbain, the amygdala and the hypothalamus. Recent studies show pancreatic intra-islet GLP-1 expression, which is regulated by cytokines, hyperglycemia and cell injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01275()