GLP/EHMT1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GLP/EHMT1 Antibody [NBP1-77400] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: GLP/EHMT1 Antibody [NBP1-77400] - IHC analysis of EMHT1 in mouse prostate using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.4 mg/ml

GLP/EHMT1 Antibody Summary

Immunogen
A genomic peptide made to an N-terminal region of the human EHMT1 protein (within residues 20-200). [Swiss-Prot Q9H9B1]
Localization
Nucleus: chromosome.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EHMT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Application Notes
This EHMT1 antibody is useful for ICC/IF and Immunohistochemistry-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for GLP/EHMT1 Antibody

  • DEL9q34
  • EC 2.1.1.-
  • EC 2.1.1.43
  • EHMT1
  • euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5
  • Eu-HMTase1
  • Eu-HMTase1DEL9q34
  • EUHMTASE1FP13812
  • FLJ12879
  • G9a-like protein 1
  • GLP
  • GLP1
  • H3 lysine-9 specific 5
  • KIAA1876euchromatic histone methyltransferase 1
  • KMT1D
  • KMT1DDKFZp667M072
  • Lysine N-methyltransferase 1D

Background

The transcription repressor EHMT1 is a histone methyltransferase that might be important in the G0/G1 cell cycle transition. In children affected with Kleefstra Syndrome, EHMT1 is mutated and seems to mis-regulate normal cellular transitions, and cause hypotonia and intellectual disability. It has been implicated in epigenetic modifications related to learning and memory formation or impairment. Finally, the translocation of this gene and erroneous protein expression have been correlated to mental retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for GLP/EHMT1 Antibody (NBP1-77400) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77400AF405
Alexa Fluor 488 Labeled NBP1-77400AF488
Alexa Fluor 647 Labeled NBP1-77400AF647
Alexa Fluor 700 Labeled NBP1-77400AF700
Biotin Labeled NBP1-77400B
DyLight 405 Labeled NBP1-77400V
DyLight 488 Labeled NBP1-77400G
DyLight 550 Labeled NBP1-77400R
DyLight 650 Labeled NBP1-77400C
FITC Labeled NBP1-77400F

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Bioinformatics

Gene Symbol EHMT1
Entrez
Uniprot