GLP/EHMT1 Antibody

Immunocytochemistry/Immunofluorescence: GLP/EHMT1 Antibody [NBP1-77400] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunohistochemistry: GLP/EHMT1 Antibody [NBP1-77400] - IHC analysis of EMHT1 in mouse prostate using DAB with hematoxylin counterstain.

Western Blot: GLP/EHMT1 Antibody [NBP1-77400] - Sequential IP in HEK293 cells demonstrating GLP/EHMT1 (NBP1-77400), G9a/EHMT2 (NBP2-13948), and LMNB1 are a part of the same complex. The dotted line indicates spliced lane from two different exposures of the same gel. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858340/) licensed under a CC-BY license.

Product Discontinued

GLP/EHMT1 Antibody Summary

• Immunogen: A genomic peptide made to an N-terminal region of the human EHMT1 protein (within residues 20-200). [Swiss-Prot Q9H9B1]
• Localization: Nucleus: chromosome.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EHMT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockdown Validated reported in scientific literature (PMID 30858340)
  • Western Blot reported in scientific literature (PMID 30858340)
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.4 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for GLP/EHMT1 Antibody

• DEL9q34
• EC 2.1.1.-
• EC 2.1.1.43
• EHMT1
• euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5
• Eu-HMTase1
• Eu-HMTase1DEL9q34
• EUHMTASE1FP13812
• FLJ12879
• G9a-like protein 1
• GLP
• GLP1
• H3 lysine-9 specific 5
• KIAA1876euchromatic histone methyltransferase 1
• KMT1D
• KMT1DDKFZp667M072
• Lysine N-methyltransferase 1D

Background

The transcription repressor EHMT1 is a histone methyltransferase that might be important in the G0/G1 cell cycle transition. In children affected with Kleefstra Syndrome, EHMT1 is mutated and seems to mis-regulate normal cellular transitions, and cause hypotonia and intellectual disability. It has been implicated in epigenetic modifications related to learning and memory formation or impairment. Finally, the translocation of this gene and erroneous protein expression have been correlated to mental retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLP/EHMT1 Antibody (NBP1-77400)(1)

Publication using NBP1-77400

• Rao RA, Ketkar AA, Kedia N, Krishnamoorthy VK, et al. KMT1 family methyltransferases regulate heterochromatin-nuclear periphery tethering via histone and non-histone protein methylation EMBO 2019-01-24 [PMID: 30858340] (WB, KD, WB, KD, Human)

Bioinformatics

• Gene Symbol: EHMT1
• Entrez:
  • 79813(Human)
• Uniprot:
  • Q9H9B1(Human)