GKAP/DLGAP1 Antibody (S127-31)

Images

 
Western Blot: GKAP/DLGAP1 Antibody (S127-31) [NBP1-47612] - analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31. Load: 15 ug protein. Block: ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
S127-31
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

GKAP/DLGAP1 Antibody (S127-31) Summary

Immunogen
Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1
Localization
Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density
Specificity
Detects approx. 120kDa (SAPAP2), approx. 110kDa (SAPAP1/3/4). It does cross-react with other SAPAPs.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DLGAP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.1-1.0 ug/ml
  • Immunohistochemistry 1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of SAPAP (pan) Antibody was sufficient for detection of SAPAP 1-4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Theoretical MW
108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-47612 in the following applications:

Reactivity Notes

Based on homology, It's predicted to detect Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GKAP/DLGAP1 Antibody (S127-31)

  • DAP1
  • DAP-1-ALPHA
  • DAP-1DAP-1-BETA
  • discs, large (Drosophila) homolog-associated protein 1
  • disks large-associated protein 1
  • DLGAP1
  • GKAP
  • GKAPMGC88156
  • hGKAP
  • PSD-95/SAP90 binding protein 1
  • PSD-95/SAP90-binding protein 1
  • SAP90/PSD-95-associated protein 1
  • SAPAP1
  • SAPAP1Guanylate kinase-associated protein

Background

Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membraneassociated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. PSD-95/SAP90 binds guanylate kinase-associated protein (GKAP), also designated GK domain-binding protein, DAP-1-alpha, DAP-1-beta, PSD-95 binding protein, PSD-95/SAP90 associated protein, or SAPAP1, through the guanylate kinase domain. SAPAP1 is expressed widely in neurons of the cortex and hippocampus and in the Purkinje and granule cells of the cerebellum. N-terminal splice variants of SAPAP1 are expressed as 95 and 130 kDa proteins. SAPAP1 is localized specifically in the PSD of glutamatergic synapses, consistent with its direct interaction with PSD-95 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-84212
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15083
Species: Hu, Rt
Applications: WB, IHC, IHC-P
AF7035
Species: Hu
Applications: IHC
NB600-1229
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-00594
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB300-167
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91031
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-713
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-91685
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP1-44999
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-47610
Species: Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-47778
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF877
Species: Hu
Applications: WB, IHC, IP
NB100-858
Species: Hu, Mu, Rt, GP, Mk
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
AF2558
Species: Hu, Mu
Applications: WB, Simple Western
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP1-47612
Species: Rt
Applications: WB, ICC/IF, IHC, IP

Publications for GKAP/DLGAP1 Antibody (NBP1-47612)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for GKAP/DLGAP1 Antibody (NBP1-47612) (0)

There are no reviews for GKAP/DLGAP1 Antibody (NBP1-47612). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GKAP/DLGAP1 Antibody (NBP1-47612) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-47612AF405
Alexa Fluor 488 Labeled NBP1-47612AF488
Alexa Fluor 647 Labeled NBP1-47612AF647
Alexa Fluor 700 Labeled NBP1-47612AF700
DyLight 350 Labeled NBP1-47612UV
DyLight 405 Labeled NBP1-47612V
DyLight 488 Labeled NBP1-47612G
DyLight 680 Labeled NBP1-47612FR
DyLight 755 Labeled NBP1-47612IR

Additional GKAP/DLGAP1 Products

Bioinformatics Tool for GKAP/DLGAP1 Antibody (NBP1-47612)

Discover related pathways, diseases and genes to GKAP/DLGAP1 Antibody (NBP1-47612). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GKAP/DLGAP1 Antibody (NBP1-47612)

Discover more about diseases related to GKAP/DLGAP1 Antibody (NBP1-47612).
 

Pathways for GKAP/DLGAP1 Antibody (NBP1-47612)

View related products by pathway.

PTMs for GKAP/DLGAP1 Antibody (NBP1-47612)

Learn more about PTMs related to GKAP/DLGAP1 Antibody (NBP1-47612).

Blogs on GKAP/DLGAP1

There are no specific blogs for GKAP/DLGAP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GKAP/DLGAP1 Antibody (S127-31) and receive a gift card or discount.

Bioinformatics

Gene Symbol DLGAP1
Entrez
Uniprot