GKAP/DLGAP1 Antibody (S127-31)

Western Blot: GKAP/DLGAP1 Antibody (S127-31) [NBP1-47612] - analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAPAP Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Discontinued

GKAP/DLGAP1 Antibody (S127-31) Summary

• Immunogen: Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1
• Localization: Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
• Specificity: Detects approx. 120kDa (SAPAP2), approx. 110kDa (SAPAP1/3/4). It does cross-react with other SAPAPs .
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLGAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.1-1.0 ug/ml
  • Immunohistochemistry 1:1000
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of SAPAP (pan) Antibody was sufficient for detection of SAPAP 1-4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
• Theoretical MW: 108 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24277719).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GKAP/DLGAP1 Antibody (S127-31)

• DAP1
• DAP-1-ALPHA
• DAP-1DAP-1-BETA
• discs, large (Drosophila) homolog-associated protein 1
• disks large-associated protein 1
• DLGAP1
• GKAP
• GKAPMGC88156
• hGKAP
• PSD-95/SAP90 binding protein 1
• PSD-95/SAP90-binding protein 1
• SAP90/PSD-95-associated protein 1
• SAPAP1
• SAPAP1Guanylate kinase-associated protein

Background

Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membraneassociated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. PSD-95/SAP90 binds guanylate kinase-associated protein (GKAP), also designated GK domain-binding protein, DAP-1-alpha, DAP-1-beta, PSD-95 binding protein, PSD-95/SAP90 associated protein, or SAPAP1, through the guanylate kinase domain. SAPAP1 is expressed widely in neurons of the cortex and hippocampus and in the Purkinje and granule cells of the cerebellum. N-terminal splice variants of SAPAP1 are expressed as 95 and 130 kDa proteins. SAPAP1 is localized specifically in the PSD of glutamatergic synapses, consistent with its direct interaction with PSD-95 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GKAP/DLGAP1 Antibody (NBP1-47612)(2)

Publications using NBP1-47612

• Grabrucker S, Jannetti L, Eckert M et al. Zinc deficiency dysregulates the synaptic ProSAP/Shank scaffold and might contribute to autism spectrum disorders. Brain. 2013-11-25 [PMID: 24277719] (ICC/IF, Mouse)
• Schaik A Resolving the effect of Shank3 S1510 phosphorylation on Shank3 functioning Thesis 2021-01-01 (IP, Human)

Bioinformatics

• Gene Symbol: DLGAP1
• Entrez:
  • 9229(Human)
  • 224997(Mouse)
  • 65040(Rat)
• Uniprot:
  • O14490(Human)
  • Q9D415(Mouse)
  • P97836(Rat)