Porcine GHRH ELISA Kit (Colorimetric)

• Reactivity: Po
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma and other biological fluids
• Standard Curve Range: 12.35 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 5.22 pg/mL (example only; lot dependent)

Porcine GHRH ELISA Kit (Colorimetric) Summary

• Description: Assay time: 2 hours
• Standard Curve Range: 12.35 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 5.22 pg/mL (example only; lot dependent)
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Spike Recovery: 86 - 97% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GHRH

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Porcine GHRH ELISA Kit (Colorimetric)

• GHRFsomatocrinin
• GRF
• growth hormone releasing hormone
• Growth hormone-releasing factor
• Growth hormone-releasing hormone
• INN
• MGC119781
• sermorelin
• Somatocrinin
• somatoliberin
• somatorelin

Background

GHRH, also known as Somatoliberin or Growth hormone-releasing hormone, has a 108 amino acid isoform and a 107 amino acid isoform that are 12 kDa, is produced by the hypothalamus and its main function is to stimulate growth hormone release. Disease research is being performed on this proteins involvement in gigantism, isolated growth hormone deficiency due to defect in ghrf, dwarfism, gigantism due to ghrf hypersecretion, zollinger-ellison syndrome, anorexia, nervosa, multiple endocrine neoplasia, polycystic ovary syndrome, short stature, Cushing's syndrome, hypopituitarism, diabetes insipidus, panic disorder, hypothyroidism, somatostatinoma, acromegaly, vipoma, pituitary adenoma, Prader-Willi syndrome, hyperprolactinemia, cancer, lipodystrophy, hypogonadism, diabetes mellitus, and Pseudohypoparathyroidism. This protein has been involved in 55 different pathways such as nuclear receptor activation by vitamin-A, Paxillin Interactions, telomerase components in cell signaling, mitochondrial apoptosis, molecular mechanisms of cancer, antioxidant action of vitamin-C, eIF2 pathway, intracellular calcium signaling, JNK pathway, apoptotic pathways in synovial fibroblasts, ERK5 signaling, Tec kinases signaling, GSK3 signaling, and cellular apoptosis pathway where it interacts with GHRHR, DPP4, FAP, ADCYAP1R1, POMC, NPS, MC4R, ADRB1, and 50 other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for GHRH ELISA Kit (NBP3-42490). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GHRH