GGT1 Overexpression Lysate

Images

 
Western Blot: GGT1 Overexpression Lysate (Adult Normal) [NBL1-11061] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GGT1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GGT1 Overexpression Lysate Summary

Description
Transient overexpression lysate of gamma-glutamyltransferase 1 (GGT1), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC223586 and based on accession number NM_005265. The protein contains a C-MYC/DDK Tag.
Gene
GGT1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GGT1 Overexpression Lysate

  • CD224 antigen
  • CD224
  • D22S672
  • D22S732
  • EC 2.3.2.2
  • gamma-glutamyl transpeptidase
  • gamma-glutamyltransferase 1MGC96904
  • gamma-glutamyltranspeptidase 1
  • GGT 1
  • GGTMGC96963
  • glutamyl transpeptidase
  • GTG
  • MGC96892

Background

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 3, 4, and 5 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GGT1 Lysate (NBL1-11061) (0)

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-11061

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Bioinformatics

Gene Symbol GGT1