GEM Overexpression Lysate (Native)

Images

 
Western Blot: GEM Overexpression Lysate (Adult Normal) [NBL1-11037] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GEM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GEM Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205286 and based on accession number NM_181702. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens GTP binding protein overexpressed in skeletal muscle (GEM), transcript variant 2, mRNA.
Gene
GEM

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GEM

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GEM Overexpression Lysate (Native)

  • GTP binding protein overexpressed in skeletal muscle
  • GTP-binding mitogen-induced T-cell protein
  • GTP-binding protein GEM
  • GTP-binding protein overexpressed in skeletal muscle
  • kinase-inducible Ras-like protein
  • KIRMGC26294
  • RAS-like protein KIR

Background

The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-29931
Species: Hu
Applications: WB, Flow, IHC-P
NBP2-50419
Species: Hu
Applications: Flow, IP, CyTOF-ready
MAB1058
Species: Hu
Applications: Flow, Block, CyTOF-ready
MAB2014
Species: Hu
Applications: Flow, CyTOF-reported
NB100-56618
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB1059
Species: Hu
Applications: Flow, CyTOF-ready
H00003126-M01
Species: Hu
Applications: WB, ELISA, IHC-P
MAB12251
Species: Hu
Applications: Flow, CyTOF-ready
MAB1844
Species: Hu
Applications: Flow, CyTOF-reported
MAB8887
Species: Hu
Applications: Flow
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
MAB2238
Species: Hu
Applications: Flow, AgAct, CyTOF-reported
NBP1-82874
Species: Hu
Applications: IHC, IHC-P
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
3015-KR
Species: Hu
NBP2-36776
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-12900
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13266
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB120-6405
Species: Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-58906
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBL1-11037
Species: Hu
Applications: WB

Publications for GEM Lysate (NBL1-11037) (0)

There are no publications for GEM Lysate (NBL1-11037).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GEM Lysate (NBL1-11037) (0)

There are no reviews for GEM Lysate (NBL1-11037). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GEM Lysate (NBL1-11037) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GEM Products

Bioinformatics Tool for GEM Lysate (NBL1-11037)

Discover related pathways, diseases and genes to GEM Lysate (NBL1-11037). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GEM Lysate (NBL1-11037)

Discover more about diseases related to GEM Lysate (NBL1-11037).
 

Pathways for GEM Lysate (NBL1-11037)

View related products by pathway.

PTMs for GEM Lysate (NBL1-11037)

Learn more about PTMs related to GEM Lysate (NBL1-11037).
 

Research Areas for GEM Lysate (NBL1-11037)

Find related products by research area.

Blogs on GEM

There are no specific blogs for GEM, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GEM Antibody
NBP1-58906

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GEM Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GEM