GDI1 Antibody

Western Blot: GDI1 Antibody [NBP2-99205] - Lane A: NIHT3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:51 kDaObserved band size:47 kDa

Immunocytochemistry/Immunofluorescence: GDI1 Antibody [NBP2-99205] - Immunofluorescence staining of GDI1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human GDI1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.

Immunohistochemistry-Paraffin: GDI1 Antibody [NBP2-99205] - Immunochemical staining of human GDI1 in human gastric cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).

Immunoprecipitation: GDI1 Antibody [NBP2-99205] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-GDI1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GDI1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size: 49 kDa

Product Discontinued

GDI1 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human GDI1.
• Predicted Species: Mouse (100%), Rat (100%), Cynomolgus Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GDI1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 4-8 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for GDI1 Antibody

• FLJ41411
• GDI-1
• GDIL
• GDP dissociation inhibitor 1
• Guanosine diphosphate dissociation inhibitor 1
• mental retardation, X-linked 41
• mental retardation, X-linked 48
• MRX41
• oligophrenin-2
• OPHN2MRX48
• Protein XAP-4
• Rab GDI alpha
• rab GDP dissociation inhibitor alpha
• rab GDP-dissociation inhibitor, alpha
• RABGDIARABGD1A
• XAP4
• XAP-4

Background

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GDI1