GCOM1 Overexpression Lysate

Western Blot: GCOM1 Overexpression Lysate (Adult Normal) [NBL1-11017] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GCOM1.

• Reactivity: Hu
• Applications: WB

GCOM1 Overexpression Lysate Summary

• Description: Transient overexpression lysate of GRINL1A complex locus (GCOM1), transcript variant 13 The lysate was created in HEK293T cells, using Plasmid ID RC215908 and based on accession number NM_152451. The protein contains a C-MYC/DDK Tag.
• Gene: GCOM1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 50.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GCOM1 Overexpression Lysate

• Gcom
• GRINL1A combined protein
• GRINL1A upstream protein
• Gup

Background

This gene (Gcom1) is part of a complex transcript unit that includes the gene for glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A). Transcription of this gene occurs at an upstream promoter, with two different groups of alternatively spliced variants: Gup for GRINL1A upstream transcripts and Gcom for GRINL1A combined transcripts. The GRINL1A gene uses a downstream promoter for transcription and also has multiple alternatively spliced variants. [provided by RefSeq]. Transcript Variant: This variant (13), also called Gup2, has multiple differences in the coding region, compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 8) has a distinct C-terminus and is shorter than isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GCOM1