| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP2-04024F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03436-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89788-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38267-25ul | |||
![Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/GCC185-Antibody-Western-Blot-NBP2-04024-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP2-04024] - PC-3 cells (human) stained with GCC185 antibody (red). Nucleus stained with DAPI (blue). EVOS(TM) M5000 Imaging System - 63x oil objective. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-04024-img0004.jpg)
![Western Blot: GCC185 Antibody [NBP3-03436] - Analysis of extracts of various cell lines, using GCC185 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GCC185-Antibody-Western-Blot-NBP3-03436-img0005.jpg "Western Blot: GCC185 Antibody [NBP3-03436] - Analysis of extracts of various cell lines, using GCC185 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded mouse spleen using GCC185 antibody at dilution of 1:150 (40x lens).](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP3-03436-img0004.jpg)
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded human colon using GCC185 antibody at dilution of 1:150 (40x lens).](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP3-03436-img0001.jpg)
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded human stomach using GCC185 antibody at dilution of 1:150 (40x lens).](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP3-03436-img0002.jpg)
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP3-03436] - Paraffin-embedded human appendix using GCC185 antibody at dilution of 1:150 (40x lens).](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP3-03436-img0003.jpg)
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP1-20922-img0003.jpg "Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - (3.8 ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP1-20922-img0001.jpg)
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP1-20922-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP1-89788] - staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89788-img0004.jpg "Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP1-89788] - staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-89788] - Staining of human colon shows distinct cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP1-89788-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP2-38267] - Staining of human cell line SiHa shows localization to nucleoplasm & the Golgi apparatus.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38267-img0002.jpg "Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP2-38267] - Staining of human cell line SiHa shows localization to nucleoplasm & the Golgi apparatus.")
![Immunohistochemistry: GCC185 Antibody [NBP2-38267] - Staining of human colon shows distinct cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/GCC185-Antibody-Immunohistochemistry-NBP2-38267-img0001.jpg)