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GATM Antibody (OTI1E3) [PE/Cy7]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
OTI1E3
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70802AF350
Alexa Fluor 405
NBP2-70802AF405
Alexa Fluor 488
NBP2-70802AF488
Alexa Fluor 532
NBP2-70802AF532
Alexa Fluor 594
NBP2-70802AF594
Alexa Fluor 647
NBP2-70802AF647
Alexa Fluor 700
NBP2-70802AF700
Alexa Fluor 750
NBP2-70802AF750
Allophycocyanin
NBP2-70802APC
Allophycocyanin/Cy7
NBP2-70802APCCY7
Biotin
NBP2-70802B
DyLight 350
NBP2-70802UV
DyLight 405
NBP2-70802V
DyLight 488
NBP2-70802G
DyLight 550
NBP2-70802R
DyLight 594
NBP2-70802DL594
DyLight 650
NBP2-70802C
DyLight 680
NBP2-70802FR
DyLight 755
NBP2-70802IR
FITC
NBP2-70802F
HRP
NBP2-70802H
Janelia Fluor 549
NBP2-70802JF549
Janelia Fluor 646
NBP2-70802JF646
PE
NBP2-70802PE
PE/Atto594
NBP2-70802PEATT594
PE/Cy5.5
NBP2-70802PECY55
PerCP
NBP2-70802PCP
Unconjugated
NBP2-00984
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00984
Azide and BSA Free
NBP2-70802
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GATM Antibody (OTI1E3) [PE/Cy7] Summary

Immunogen
Full length recombinant protein of human GATM (NP_001473) produced in HEK293T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GATM
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GATM Antibody (OTI1E3) [PE/Cy7]

  • AGATtransamidinase
  • AT
  • EC 2.1.4
  • EC 2.1.4.1
  • glycine amidinotransferase (L-arginine:glycine amidinotransferase)
  • glycine amidinotransferase, mitochondrial
  • L-arginine:glycine amidinotransferase
  • Transamidinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GAMT Antibody
NBP2-14036
Western Blot: GAMT Antibody [NBP2-14036] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: GAMT Antibody [NBP2-14036] - Staining of human rectum shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: GAMT Antibody [NBP2-14036] - Staining of human liver shows weak to moderate cytoplasmic postivity in hepatocytes na moderate staining of bile canaliculi.
Species: Hu
Applications: WB, IHC, IHC-P
SLC6A8 Antibody
NBP2-11567
Western Blot: SLC6A8 Antibody [NBP2-11567] - Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/ml, 1 hr incubation. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SLC6A8 Antibody [NBP2-11567] - Human kidney. Antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH6, AP-staining.
(1 Publication)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
CD55/DAF Antibody
NBP1-85466
Immunohistochemistry-Paraffin: CD55/DAF Antibody [NBP1-85466] - Staining in human lung and kidney tissues using anti-CD55 antibody. Corresponding CD55 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD55/DAF Antibody [NBP1-85466] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: CD55/DAF Antibody [NBP1-85466] - Staining of human lung shows high expression.
(2 Publications)
Species: Hu, Mu, Fe
Applications: WB, IHC, IHC-P
OAT Antibody
NBP1-83239
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-83239] - Analysis in human duodenum and pancreas tissues. Corresponding OAT RNA-seq data are presented for the same tissues.
Western Blot: OAT Antibody [NBP1-83239] - Analysis in control (vector only transfected HEK293T lysate) and OAT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-83239] - Staining of human duodenum shows strong granular cytoplasmic postivity in glandular cells.
(2 Reviews)
Species: Hu, Bv
Applications: WB, IHC, IHC-P
DHRS4 Antibody
NBP2-98675
Western Blot: DHRS4 Antibody [NBP2-98675] - Anti-DHRS4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa
Immunohistochemistry-Paraffin: DHRS4 Antibody [NBP2-98675] - Immunochemical staining of human DHRS4 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: DHRS4 Antibody [NBP2-98675] - DHRS4 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate 4 ul anti-DHRS4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DHRS4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa
Species: Hu
Applications: WB, IHC, IHC-P, IP
CRIPTO Antibody
NB100-1598
Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
(3 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
Calretinin Antibody
NBP1-88220
Western Blot: Calretinin Antibody [NBP1-88220] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-88220] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining in human testis and skeletal muscle tissues using NBP1-88220 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
CD21 Antibody
(SC0681)
NBP2-67605
Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on different lysates using anti-CD21 antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: Raji
Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
LYAG/GAA Antibody
NBP2-38142
Western Blot: LYAG/GAA Antibody [NBP2-38142] - Analysis in control (vector only transfected HEK293T lysate) and GAA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142] - Staining of human prostate shows moderate positivity in lysosomes in glandular cells.
Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142] - Analysis in human placenta and tonsil tissues. Corresponding GAA RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, IHC, IHC-P

Cardiotrophin-1/CT-1
612-CD/CF
Cardiotrophin-1/CT-1
(7 Publications)
Species: Hu
Growth Hormone
Antibody [Unconjugate
AF1067
Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50 is typically 1.5-7.5 ng/mL.
(3 Reviews)
(6 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
alpha-Defensin 1
Antibody [Unconjugate ...
AF8198
Recombinant Human alpha ‑Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha ‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human alpha ‑Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
alpha ‑Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human alpha ‑Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: ELISA, IHC
Methionyl tRNA
synthetase Antibody
NBP1-87730
Western Blot: Methionyl tRNA synthetase Antibody [NBP1-87730] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of human testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
(13 Publications)
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P
HPRT Antibody
NBP1-33527
Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
ME3 Antibody
NBP1-85871
Western Blot: ME3 Antibody [NBP1-85871] - Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: ME3 Antibody [NBP1-85871] - Staining of human tonsil shows very weak granular cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: ME3 Antibody [NBP1-85871] - Staining of human endometrium shows moderate granular cytoplasmic positivity.
Species: Hu
Applications: WB, IHC, IHC-P
GLYAT Antibody
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human skeletal muscle.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.
Species: Hu
Applications: WB, IHC, IHC-P
ME1 Antibody
NBP1-32398
Western Blot: ME1 Antibody [NBP1-32398] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ME1 Antibody [NBP1-32398] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-32398] - Paraffin-embedded human ovarian carcinoma. ME1 antibody dilution: 1:500.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70802AF350
Alexa Fluor 405 NBP2-70802AF405
Alexa Fluor 488 NBP2-70802AF488
Alexa Fluor 532 NBP2-70802AF532
Alexa Fluor 594 NBP2-70802AF594
Alexa Fluor 647 NBP2-70802AF647
Alexa Fluor 700 NBP2-70802AF700
Alexa Fluor 750 NBP2-70802AF750
Allophycocyanin NBP2-70802APC
Allophycocyanin/Cy7 NBP2-70802APCCY7
Biotin NBP2-70802B
DyLight 350 NBP2-70802UV
DyLight 405 NBP2-70802V
DyLight 488 NBP2-70802G
DyLight 550 NBP2-70802R
DyLight 594 NBP2-70802DL594
DyLight 650 NBP2-70802C
DyLight 680 NBP2-70802FR
DyLight 755 NBP2-70802IR
FITC NBP2-70802F
HRP NBP2-70802H
Janelia Fluor 549 NBP2-70802JF549
Janelia Fluor 646 NBP2-70802JF646
PE NBP2-70802PE
PE/Atto594 NBP2-70802PEATT594
PE/Cy5.5 NBP2-70802PECY55
PE/Cy7 NBP2-70802PECY7
PerCP NBP2-70802PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol GATM