GASP-2/WFIKKN Antibody (289839) [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clone: 289839
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

GASP-2/WFIKKN Antibody (289839) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GASP-2/WFIKKN
  Ala20-Asp548
  Accession # Q96NZ8
• Specificity: Detects human GASP‑2/WFIKKN in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human GASP-1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: WFIKKN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human GASP-2/WFIKKN Antibody (Catalog # MAB21361)
  ELISA Detection: Human GASP-2/WFIKKN Biotinylated Antibody (Catalog # BAM21362)
  Standard: Recombinant Human GASP-2/WFIKKN (Catalog # 2136-GS)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GASP-2/WFIKKN Antibody (289839) [Biotin]

• C16orf12
• Chromosome 16 Open Reading Frame 12
• GASP2
• GASP-2
• Growth And Differentiation Factor-Associated Serum Protein 2
• hGASP-2
• RJD2
• WAP Four-Disulfide Core Domain 20A
• WAP, Follistatin/Kazal, Immunoglobulin, Kunitz And Netrin Domain Containing 1
• WAP, FS, Ig, KU, And NTR-Containing Protein
• WFDC20A
• WFIKKN
• WFIKKN1

Background

Growth and differentiation factor-associated serum protein-2 (GASP-2) cDNA encodes a 548 amino acid protein that contains a 19 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Based on the order of these conserved domains, GASP-2 is also known as WFIKKN (1). Another related protein which contains the same domain structure is called WFIKKNRP (WFIKKN-related protein), or GASP-1 (2, 3). WAP, follistatin, Kunitz and netrin domains are all implicated in protease inhibition, and the GASP proteins may be multivalent protease inhibitors (1, 4). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-2 is abundant in the lung, skeletal muscle and liver while GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney (3). In the adult, GASP-2 is expressed primarily in the pancreas, liver, and thymus while GASP-1 is in the ovary, testis, and brain (3). Further characterization shows that GASP-1 inhibits myostatin (GDF-8) and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (2). Although, this kind of activity has not been reported for GASP-2, tests at R&D Systems have determined that GASP-2 shows similar inhibitory activity towards myostatin as GASP-1. By amino acid sequence, human GASP-2 is 55% identical to human GASP-1.

1. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3705.
2. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
3. Trexler, M. et al. (2002) Biol. Chem. 383:223.
4. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WFIKKN1
• Uniprot:
  • Q96NZ8()