GAS7 Antibody (OTI2F6) - Azide and BSA Free

Western Blot: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7.

Immunocytochemistry/Immunofluorescence: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GAS7.

Immunohistochemistry: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GAS7 mouse monoclonal antibody.

Flow Cytometry: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GAS7 antibody, and then analyzed by flow cytometry.

Western Blot: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GAS7 monoclonal antibody.

Immunohistochemistry: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Staining of paraffin-embedded Human Kidney tissue using anti-GAS7 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI2F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GAS7 Antibody (OTI2F6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GAS7 Antibody (OTI2F6) - Azide and BSA Free (NBP2-03061) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human GAS7 (NP_003635) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAS7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GAS7 Antibody (OTI2F6) - Azide and BSA Free

• GAS-7
• growth arrest-specific 7
• KIAA0394growth arrest-specific protein 7
• MGC1348
• MLL/GAS7 fusion protein
• MLL/GAS7

Background

Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAS7