gamma H2AX [p Ser139] Antibody (9F3)

Images

 
Western Blot: gamma H2AX [p Ser139] Antibody (9F3) [NBP1-19255] - Lane 1: MWM, Lane 2: Jurkak, Lane 3: Jurkat staurosporine, Lane 4: 3T3, Lane 5: CHO-K1, Lane 6: Rat-2

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Pm, Rb, ShSpecies Glossary
Applications WB
Clone
9F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

gamma H2AX [p Ser139] Antibody (9F3) Summary

Immunogen
Synthetic phospho-peptide derived from the sequence of human Histone H2AX phosphorylated at Ser139.
Modification
p Ser139
Marker
DNA Double-strand break marker
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
H2AFX
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
Detects a band of ~16kDa by Western blot.
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for gamma H2AX [p Ser139] Antibody (9F3)

  • H2A histone family, member X
  • H2A.X
  • H2a/x
  • H2AX histone
  • H2AXhistone H2A.x
  • YH2AX

Background

H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol H2AFX
Entrez
Uniprot