gamma Catenin Antibody (JF0973)

Western Blot: gamma Catenin Antibody (JF0973) [NBP2-66858] - Western blot analysis of gamma Catenin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: human skin tissue lysate

Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (JF0973) [NBP2-66858] - Staining gamma Catenin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: gamma Catenin Antibody (JF0973) [NBP2-66858] - Analysis of paraffin-embedded mouse stomach tissue using anti-gamma Catenin antibody. Counter stained with hematoxylin.

Flow Cytometry: gamma Catenin Antibody (JF0973) [NBP2-66858] - Analysis of Hela cells with gamma Catenin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Western Blot: gamma Catenin Antibody (JF0973) [NBP2-66858] - Analysis of gamma Catenin on Hela cells lysates using anti-gamma Catenin antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (JF0973) [NBP2-66858] - Staining gamma Catenin in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (JF0973) [NBP2-66858] - Staining gamma Catenin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

gamma Catenin Antibody (JF0973) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human gamma Catenin aa 10-116 / 745. (SwissProt: P14923 Human; SwissProt: Q02257 Mouse; SwissProt: Q6P0K8 Rat)
• Localization: Cytoskeleton, adherens junction, desmosome, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: JUP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for gamma Catenin Antibody (JF0973)

• catenin (cadherin-associated protein), gamma (80kD)
• catenin (cadherin-associated protein), gamma 80kDa
• Catenin gamma
• Desmoplakin III
• desmoplakin-3
• DP3CTNNG
• DPIII
• gamma-catenin
• junction plakoglobin
• PDGBARVD12
• PKGB

Background

Catenin gamma, also known as Junction Plakoglobin, is a common junctional plaque protein. It is a cytoplasmic protein with soluble and membrane-associated forms. The presence of Catenin gamma in both the desmosomes and in the intermediate junctions suggests it has a role in the structure and Function of submembranous plaques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JUP