Galectin-3 Antibody (194801) [Phycoerythrin]

LPS-treated peripheral blood monocytes were stained with Mouse Anti-Human Galectin-3 PE-conjugated Monoclonal Antibody (Catalog # IC11542P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 194801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Galectin-3 Antibody (194801) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human Galectin-3
  Ala2-Ile250
  Accession # P17931
• Specificity: Detects human Galectin-3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Galectin-3 is observed and no cross-reactivity with recombinant human (rh) Galectin-8 or rhGalectin-9 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGALS3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-3 Antibody (194801) [Phycoerythrin]

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

Galectin-3, also known as Mac-2, L29, CBP35 and IgE Binding Protein, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their Carbohydrate Recognition Domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts where it forms homodimers, or heterodimers, likely with DMBT1. It is reported to complex with Integrins alpha3 and beta1. Galectin-3 is known to circulate and show elevation in inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGALS3
• Uniprot:
  • P17931(Mouse)