Recombinant Human Galectin-10 His Protein

SDS-Page: Recombinant Human Galectin-10 Protein [NBP1-51096]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Galectin-10 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-142 of Human Galectin-10
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI VFHFQVCFGR RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY QVMVNGQSSY TFDHRIKPEA VKMVQVWRDI SLTKFNVSYL KR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CLC
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Galectin-10 His Protein

• Charcot-Leyden crystal proteinLGALS10A
• CLC
• EC 3.1.1.5
• eosinophil lysophospholipase
• GAL10
• Gal-10
• galectin 10
• Galectin10
• Galectin-10
• LGALS10
• LPPL_HUMAN
• Lysolecithin acylhydrolase
• MGC149659

Background

Eosinophil lysophospholipase (CLC), also known as Galectin 10, act on biological membranes to regulate the multifunctional lysophospholipids. CLC is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias. Recombinant human CLC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Galectin-10 Recombinant Protein (NBP1-51096)(4)

Publications using NBP1-51096

• Willems M Eosinophils inhibit response to chemotherapy: mechanisms, preclinical and clinical study in mesothelioma Thesis 2023-01-01 (FLOW, Human)
• Willems, M;Hamaidia, M;Fontaine, A;Grégoire, M;Halkin, L;Vilanova Mañá, L;Terres, R;Jamakhani, M;Deshayes, S;Brostaux, Y;Heinen, V;Louis, R;Duysinx, B;Jean, D;Wasielewski, E;Scherpereel, A;Blanquart, C;Willems, L; The impact of Charcot-Leyden Crystal protein on mesothelioma chemotherapy: targeting eosinophils for enhanced chemosensitivity EBioMedicine 2024-10-28 [PMID: 39471751]
• Lu Y, Huang Y, Li J Et al. Eosinophil extracellular traps drive asthma progression through neuro-immune signals Nature cell biology 2021-10-01 [PMID: 34616019] (In vitro)
• Lingblom C, Andersson J, Andersson K, Wenneras C. Regulatory Eosinophils Suppress T Cells Partly through Galectin-10. J. Immunol. 2017-05-17 [PMID: 28515279] (FLOW, Human)

Bioinformatics

• Gene Symbol: CLC