Galanin R1/GALR1 Antibody

Immunohistochemistry-Paraffin: GALR1 Antibody [NLS4105] - Formalin-fixed paraffin section of human brain, stained (20 ug/ml)

Product Discontinued

Galanin R1/GALR1 Antibody Summary

• Immunogen: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human GALR1.
• Localization: Cell Membrane; multi pass membrane protein
• Specificity: Human GALR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SSTR5 (50%).
• Predicted Species: Primate (94%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GALR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 19 ug/ml
• Application Notes: ICC/IF usage is reported in scientific literature (PMID: 21821102).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (94%), Canine (89%), Panda (83%), Equine (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Galanin R1/GALR1 Antibody

• GAL1
• Galanin R1
• galanin receptor 1
• galanin receptor type 1
• GalaninR1
• GALNR1
• GALNRGALNR1GAL1-R
• GALR1
• GALR-1

Background

GalR1 is a Galanin Receptor that mediates the effects of the peptide galanin in a wide variety of tissues. Unlike GalR2, the primary signaling mechanism of GalR1 is by inhibiting adenylate cyclase through Gi. GalR1 regulates the GnRH neuroendocrine axis with its expression in POMC neurons. Pathogens like E. coli can cause diarrhea by increasing expression of Galanin Receptor 1 in the epithelial cells lining the colon, resulting in increased secretion of colonocyte chloride and fluid. GalR1 has been reported in brain, spinal cord, small intestine, colon, and eye. ESTs have been isolated from nerve and fetal lung/testis/B-cell libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galanin R1/GALR1 Antibody (NLS4105)(1)

Publication using NLS4105

• Barson JR, Chang G-Q, Poon K et al. Galanin and the orexin 2 receptor as possible regulators of enkephalin in the paraventricular nucleus of the hypothalamus: relation to dietary fat. Neuroscience. 2011-07-28 [PMID: 21821102]

FAQs for Galanin R1/GALR1 Antibody (NLS4105). (Showing 1 - 1 of 1 FAQ).

1. Could I use GALR1 Antibody in frog brain tissue?
   • Unfortunately we have never tested in frog tissue before so the chances of it cross reacting would depend on the protein sequence and how similar it is with the peptide used to synthesize this antibody. If you know the sequence I can see if we can do an alignment and let you know. Otherwise, you can use our Innovators Reward Program.

Bioinformatics

• Gene Symbol: GALR1
• Entrez:
  • 2587(Human)
• Uniprot:
  • P47211()