Galanin R1/GALR1 Antibody (613207) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human Galanin R1/GALR1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Galanin R1/GALR1 Monoclonal Antibody (Catalog # MAB10353) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 613207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Galanin R1/GALR1 Antibody (613207) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Galanin R1/GALR1
  Accession # P47211
• Specificity: Detects human Galanin R1/GALR1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galanin R1/GALR1 Antibody (613207) [Unconjugated]

• GAL1
• Galanin R1
• galanin receptor 1
• galanin receptor type 1
• GalaninR1
• GALNR1
• GALNRGALNR1GAL1-R
• GALR1
• GALR-1

Background

The neuropeptide galanin elicits a range of biological effects by interaction with specific G protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P47211()