GADD45G Antibody

Product Discontinued

GADD45G Antibody Summary

• Immunogen: Peptide with sequence C-RMQGAGKALHE corresponding to internal region according to NP_006696.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GADD45G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 23 kDa band in human testis and thyroid gland lysates and in lysates of A549 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1 kDa band according to NP_006696.1. The 23 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  GADD45G Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for GADD45G Antibody

• CR6DNA damage-inducible transcript 2 protein
• Cytokine-responsive protein CR6
• DDIT-2
• DDIT2GADD45-gamma
• growth arrest and DNA damage-inducible protein GADD45 gamma
• growth arrest and DNA-damage-inducible, gamma
• GRP1717 kD

Background

GADD45G is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GADD45G Antibody (NBP1-00189)(1)

Publication using NBP1-00189

• Zerbini LF, Wang Y, Czibere A et al. NF-kappa B-mediated repression of growth arrest- and DNA-damage-inducibleproteins 45alpha and gamma is essential for cancer cell survival. Proc Natl Acad Sci U S A 2004-09-14 [PMID: 15353598]

Bioinformatics

• Gene Symbol: GADD45G
• Entrez:
  • 10912(Human)
  • 23882(Mouse)
  • 291005(Rat)
• Uniprot:
  • NP_006696.1(Human)