GAD65/67 Antibody

Product Discontinued

GAD65/67 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 540-590 of Human GAD-65.
• Marker: GABAergic Neuronal Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of GAD65/67 (D572) pAb in extracts from mouse brain.
  Immunohistochemistry (IHC) analyzes of GAD65/67 (D572) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GAD65/67 Antibody

• 67kD)
• EC 4.1.1
• FLJ45882
• glutamate decarboxylase 1 (brain, 67kDa)
• Glutamate decarboxylase 67 kDa isoform

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAD65/67 Antibody (NB100-92033)(1)

Publication using NB100-92033

• Merlini M, Rafalski VA, Ma K et al. Microglial Gi-dependent dynamics regulate brain network hyperexcitability Nature neuroscience 2021-01-01 [PMID: 33318667]

Bioinformatics

• Gene Symbol: GAD1
• Entrez:
  • 2571(Human)