GAD1/GAD67 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Immunocytochemical staining of GAD1/GAD67 (RED/YELLOW) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD1/GAD67 staining was ...read more
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD1/GAD67 (GREEN) immunoreactivity. Phalloidin (F-actin) (RED) staining. ...read more
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Immunohistochemical staining of inhibitory neurons (RED) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

GAD1/GAD67 Antibody Summary

Immunogen
Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the C-terminus of GAD1/GAD67, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products.
Marker
Neuron Cell Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
GAD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:500-1:1000
Application Notes
This antibody preparation was analyzed by immunohistochemistry (1 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500) as the secondary reagent.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-02161.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Notes

Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized through a 0.45 um filter.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for GAD1/GAD67 Antibody

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GAD1
Entrez
Uniprot