GAD1/GAD67 Antibody

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Western Blot: GAD1/GAD67 Antibody [NB100-56385] - Analysis of GAD67 in SKNSH cell lysate using this antibody at 0.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Fe, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GAD1/GAD67 Antibody Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of amino acids 1-100 of human GAD67. The sequence used is 100% homologous with mouse and 94% homologous with rat GAD67.
Localization
Cytoplasm
Predicted Species
Mouse (94%), Rat (94%), Porcine (94%), Bovine (94%), Feline (94%), Canine (94%), Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAD1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
Positive Control
GAD1/GAD67 Lysate (NBL1-10933)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for GAD1/GAD67 Antibody

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

Principal Names: GAD1; Glutamate Decarboxylase 1; GAD67; Glutamic acid Decarboxylase 67; Official Gene Symbol: GAD1 Gene ID: 2571 (Human) Gene Map Locus: 2q31 (human) Glutamate Decarboxylase (EC 4.1.1.15) catalyzes the conversion of L-glutamic acid to GABA, a major inhibitory neurotransmitter in the CNS. GAD67, a 67KDa protein, is a neuronal isoform of this enzyme predominantly expressed in human Brain. It consists of Pyridoxal 5'-phosphate binding site and conserved Cysteine residues which are vital for its activity and is inhibited due to phosphorylation. It has high binding affinity for the cofactor Pyridoxal-5'phosphate and its role has been implicated in normal Palate development. It is found to be associated with childhood onset schizophrenia and craniofacial malformations and has been identified as a major auto antigen and an auto-reactive T-cell target in IDDM. Deficiency of this enzyme leads to pyridoxine dependency along with epilepsy. It also might play a role in stiff main syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GAD1
Entrez
OMIM
Uniprot