GAD1/GAD67 Antibody

Western Blot: GAD1/GAD67 Antibody [NB100-56385] - Analysis of GAD67 in SKNSH cell lysate using this antibody at 0.5 ug/ml.

Product Discontinued

GAD1/GAD67 Antibody Summary

• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of amino acids 1-100 of human GAD67. The sequence used is 100% homologous with mouse and 94% homologous with rat GAD67.
• Localization: Cytoplasm
• Predicted Species: Mouse (94%), Rat (94%), Porcine (94%), Bovine (94%), Feline (94%), Canine (94%), Primate (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/ml
• Control:

  GAD1/GAD67 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for GAD1/GAD67 Antibody

• 67kD)
• EC 4.1.1
• FLJ45882
• GAD1
• GAD25
• GAD67
• glutamate decarboxylase 1 (brain, 67kDa)
• Glutamate decarboxylase 67 kDa isoform

Background

Principal Names: GAD1; Glutamate Decarboxylase 1; GAD67; Glutamic acid Decarboxylase 67; Official Gene Symbol: GAD1 Gene ID: 2571 (Human) Gene Map Locus: 2q31 (human) Glutamate Decarboxylase (EC 4.1.1.15) catalyzes the conversion of L-glutamic acid to GABA, a major inhibitory neurotransmitter in the CNS. GAD67, a 67KDa protein, is a neuronal isoform of this enzyme predominantly expressed in human Brain. It consists of Pyridoxal 5'-phosphate binding site and conserved Cysteine residues which are vital for its activity and is inhibited due to phosphorylation. It has high binding affinity for the cofactor Pyridoxal-5'phosphate and its role has been implicated in normal Palate development. It is found to be associated with childhood onset schizophrenia and craniofacial malformations and has been identified as a major auto antigen and an auto-reactive T-cell target in IDDM. Deficiency of this enzyme leads to pyridoxine dependency along with epilepsy. It also might play a role in stiff main syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAD1
• Entrez:
  • 2571(Human)
  • 14415(Mouse)
  • 24379(Rat)
• OMIM:
  • 603513(Human)
• Uniprot:
  • Q99259()