GABPB1 Overexpression Lysate

Images

 
Western Blot: GABPB1 Overexpression Lysate (Adult Normal) [NBL1-10913] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GABPB1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GABPB1 Overexpression Lysate Summary

Description
Transient overexpression lysate of GA binding protein transcription factor, beta subunit 1 (GABPB1), transcript variant beta-2 The lysate was created in HEK293T cells, using Plasmid ID RC206260 and based on accession number NM_016654. The protein contains a C-MYC/DDK Tag.
Gene
GABPB1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
41.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GABPB1 Overexpression Lysate

  • E4TF1
  • E4TF1-47
  • E4TF1-53
  • E4TF1BBABPB2
  • GA binding protein transcription factor, beta subunit 1
  • GA binding protein transcription factor, beta subunit 2
  • GA-binding protein subunit beta-1
  • GABP subunit beta-1
  • GABPB-1
  • GABPB2
  • GABPB-2
  • GABPBGABP subunit beta-2
  • NRF2B1
  • NRF2B2
  • Nuclear respiratory factor 2
  • Transcription factor E4TF1-47
  • Transcription factor E4TF1-53

Background

This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (beta 2) lacks an alternate in-frame segment, compared to variant beta 1, resulting in a shorter isoform (beta 2) that lacks a 12-amino acid serine-rich region, compared to isoform beta 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GABPB1 Lysate (NBL1-10913) (0)

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Video Protocols

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Other Available Formats

NBL1-10913

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Bioinformatics

Gene Symbol GABPB1
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