GABPB1 Antibody

Western Blot: GABPB1 Antibody [NB100-60948] - Using 0.3 ug/ml for the staining of HeLa cell lysate (A) + peptide (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Goat Polyclonal GABPB1 Antibody [NB100-60948] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear/nuclear speckle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/Immunofluorescence: Goat Polyclonal GABPB1 Antibody [NB100-60948] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: Goat Polyclonal GABPB1 Antibody [NB100-60948] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948] - Using 3.75 ug/ml for the staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: GABPB1 Antibody [NB100-60948] - Using 3.75 ug/ml for the staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GABPB1 Antibody Summary

• Immunogen: Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2; NP_002032.2; NP_001307844.1.
• Epitope: SSENSSKATDETG
• Specificity: This antibody is expected to recognise isoform beta 1 (NP_005245.2), isoform gamma 1 (NP_002032.2), and beta-1 isoform 5 NP_001307844.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GABPB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection limit 1:32000
  • Flow Cytometry 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Western Blot 0.3-1 ug/ml
• Application Notes: WB: Approx. 45+38 kDa band bands observed in HeLa lysates (calculated MW of 42.5 kDa band according to NP_005245.2, P_001307844.1, and of 38.1 kDa band according to NP_002032.2). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  GABPB1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GABPB1 Antibody

• E4TF1
• E4TF1-47
• E4TF1-53
• E4TF1BBABPB2
• GA binding protein transcription factor, beta subunit 1
• GA binding protein transcription factor, beta subunit 2
• GA-binding protein subunit beta-1
• GABP subunit beta-1
• GABPB-1
• GABPB2
• GABPB-2
• GABPBGABP subunit beta-2
• NRF2B1
• NRF2B2
• Nuclear respiratory factor 2
• Transcription factor E4TF1-47
• Transcription factor E4TF1-53

Background

This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (beta 1) represents the longest transcript and encodes the longest isoform (beta 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABPB1 Antibody (NB100-60948)(1)

Publication using NB100-60948

• O'Leary DA, Koleski D, Kola I et al. Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1. Gene 2005-01-03 [PMID: 15656975]

Bioinformatics

• Gene Symbol: GABPB1
• Entrez:
  • 126626(Human)
• Uniprot:
  • NP_001307844.1(Human)
  • NP_002032.2(Human)
  • NP_005245.2(Human)