GABA-B R2 [p Ser783] Antibody - Azide Free

Western Blot: GABA-B R2 [p Ser783] Antibody [NB100-61101] - Rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch, Pm, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GABA-B R2 [p Ser783] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit GABA-B R2 [p Ser783] Antibody - Azide Free (NB100-61101) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH. Accession # O88871
• Modification: p Ser783
• Specificity: Specific for endogenous levels of the ~102 kDa GABA-B R2 phosphorylated at Ser783. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Canine (100%), Chicken (100%), Xenopus (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABBR2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:500
  • Western Blot 1:1000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GABA-B R2 [p Ser783] Antibody - Azide Free

• G protein-coupled receptor 51
• GABA B R2
• GABABR2
• GABA-BR2
• GABA-B-R2
• GABABR2GABA-B receptor 2
• GABBR2
• gamma-aminobutyric acid (GABA) B receptor, 2
• gamma-aminobutyric acid type B receptor subunit 2
• gb2
• GPR51
• GPR51GABA-B receptor, R2 subunit
• GPRC3B
• GPRC3BFLJ36928
• G-protein coupled receptor 51
• HG20HRIHFB2099

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for GABA-B R2 Antibody (NB100-61101). (Showing 1 - 1 of 1 FAQ).

1. Are there neutralizing peptides for this antibody?
   • There is no neutralizing peptide available for this antibody.

Bioinformatics

• Gene Symbol: GABBR2
• Entrez:
  • 25451(Rat)
• Uniprot:
  • O88871()