GABA-B R2 Antibody (2605F) [Unconjugated]

Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse/Rat GABAB R2 Monoclonal Antibody (Catalog # MAB104561) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for GABAB R2 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows lysates of rat brain tissue, loaded at 0,.2 mg/mL. A specific band was detected for GABAB R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Mouse/Rat GABAB R2 Monoclonal Antibody (Catalog # MAB104561) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Mu, Rt
• Applications: WB, Simple Western
• Clone: 2605F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GABA-B R2 Antibody (2605F) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat GABA_B R2
  Trp41-Ser482
  Accession # O88871
• Specificity: Detects rat GABA_B R2 in direct ELISAs. Detects mouse and rat GABA_B R2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from egg yolks

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GABA-B R2 Antibody (2605F) [Unconjugated]

• G protein-coupled receptor 51
• GABA B R2
• GABABR2
• GABA-BR2
• GABA-B-R2
• GABABR2GABA-B receptor 2
• GABBR2
• gamma-aminobutyric acid (GABA) B receptor, 2
• gamma-aminobutyric acid type B receptor subunit 2
• gb2
• GPR51
• GPR51GABA-B receptor, R2 subunit
• GPRC3B
• GPRC3BFLJ36928
• G-protein coupled receptor 51
• HG20HRIHFB2099

Background

GABBR2 is half of a heterodimeric GPCR for GABA which mediates coupling to G proteins. B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase As a homodimer, GABBR2 is retained in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartment.  When GBR1/GBR2 heterodimers are present they are localized to the plasma membrane. GABBR2 is differentially expressed in the nervous system. GABBR2 and GABBR1 mRNA's are coexpressed in various brain regions such as the Purkinje cell layer of the cerebellum. In situ hybridization histochemistry using an antisensce probe to this novel receptor indicates GABBR2 is found exclusively in neurons. GABBR2 is implicated in synaptic inhibition, hippocampal long-term potentiation, slow wave sleep, muscle relaxation and nociception.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O88871()