GABA-B R1 Antibody (751216) [Unconjugated]

GABAB R1 was detected in perfusion fixed frozen sections of rat trigeminal ganglia using Mouse Anti-Rat GABAB R1 Mono­clonal Antibody (Catalog # MAB7000) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm of sensory neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: IHC
• Clone: 751216
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GABA-B R1 Antibody (751216) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant rat GABA_B R1
  Gly17-Leu586
  Accession # Q9Z0U4
• Specificity: Detects rat GABA_B R1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABBR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GABA-B R1 Antibody (751216) [Unconjugated]

• FLJ92613
• GABA B R1
• GABABR1
• GABBR1
• GABBR1-3
• gamma-aminobutyric acid (GABA) B receptor, 1
• gamma-aminobutyric acid type B receptor subunit 1
• Gb1
• GPRC3A
• GPRC3Asubunit 1c
• hGB1a
• seven transmembrane helix receptor

Background

GABA_B R1 (GABA-B receptor subunit 1; also GABA-BR1, GABBR1 and GB1) is a multispan glycoprotein in the GABA-B receptor subfamily, GPCR-3 family of proteins. It forms an obligatory heterodimer with GABA-BR2, creating a G-protein metabotropic GABA receptor that inhibits adenylyl cyclase activity and activates K⁺ channels. Presynaptically, this blocks neurotransmitter release; postsynaptically, it lowers neuron excitability. Rat GABA_B R1 is 991 amino acids (aa) in length. It contains a 16 aa signal sequence, an extended N‑terminal extracellular region (aa 17-590) that contains two SUSHI domains (aa 29‑158), and a long C-terminal cytoplasmic domain (aa 885-991). There are several splice variants with predicted molecular weights ranging from 90 to 111 kDa and multiple glycosylation sites. The 991 aa isoform described above is called GABA_B R1e (R1e). There is also a 960 aa, 130 kDa isoform that shows a deletion of aa 771‑801. This variant (R1a) is associated with postsynaptic membranes. A third isoform (R1b) is 844 aa in length and 100 kDa in size and possesses both a deletion of aa 771-801 and a 47 aa substitution for aa 1-163. This variant is presynaptic in location. Two other isoforms are variants of GABA_B R1b. Each show the same N‑terminal substitution, with a fourth isoform (R1c) retaining aa 771-801 and a fifth isoform (R1d) deleting aa 771-801, coupled to a 25 aa substitution for aa 935‑991. Over aa 17-586, rat GABA_B R1e/a shares 99% aa identity with both mouse and human GABA_B R1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABBR1
• Uniprot:
  • Q9Z0U4(Mouse)