GABA-A R beta 1 Antibody [Unconjugated] - C-terminus

Western blot of 7 μg of rat cerebellar and hippocampal lysates showing specific immunolabeling of the ~55 kDa beta 1 subunit of the GABAA R.

Product Discontinued

GABA-A R beta 1 Antibody [Unconjugated] - C-terminus Summary

• Immunogen: Fusion protein from the cytosolic loop of the rat GABA_A R beta 1 subunit, C-Terminus
• Specificity: Human, mouse, and rat ~55 kDa GABA_A R beta 1 subunit, C-Terminus
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
• Buffer: 100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GABA-A R beta 1 Antibody [Unconjugated] - C-terminus

• GABA A R beta 1
• GABA(A) receptor subunit beta-1
• GABAAR beta 1
• GABAARb1
• GABRB1
• gamma-aminobutyric acid (GABA) A receptor, beta 1
• gamma-aminobutyric acid receptor subunit beta-1

Background

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha , beta , gamma , δ, epsilon , theta , and π. Three subunits, alpha , beta , and gamma , have at least three separate gene products in mammals, and typical GABA_A receptors have some combination of alpha , beta and gamma subunits. The rat beta 1 isoform is a 58 - 60 kDa, 449 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 13 - 218). The beta 1 subunit is known to be phosphorylated on a consensus phosphorylation site (S409 of the precursor) that exists in the cytoplasmic domain between transmembrane segments 3 and 4. PKA or PKC beta II-induced phosphorylation decreases GABA_A receptor activity.

1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
4. Hinkle, D.J. and R.L. MacDonald (2003) J. Neurosci. 23:11698.
5. Brandon, N.J. et al. (2003) Mol. Cell. Neurosci. 22:87.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABRB1