GAB2 Overexpression Lysate (Native)

Images

 
Western Blot: GAB2 Overexpression Lysate (Adult Normal) [NBP2-04413] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GAB2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GAB2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_536739. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens GAB2, mRNA.
Gene
GAB2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
74.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GAB2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GAB2 Overexpression Lysate (Native)

  • GAB2
  • GRB2-associated binding protein 2
  • GRB2-associated-binding protein 2
  • Growth factor receptor bound protein 2-associated protein 2
  • KIAA0571GRB2-associated binder 2
  • p100
  • pp100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-54602
Species: Vi
Applications: WB, ELISA, Flow, ICC/IF
MAB38461
Species: Hu
Applications: WB, IHC
NBP2-37742
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
NBP2-30691
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-46403
Species: Hu
Applications: IHC
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-46404
Species: Hu
Applications: IHC, IHC-P
NBP1-88650
Species: Hu
Applications: WB, IHC, IHC-P
H00009139-M15
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-22212
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC
MAB6777
Species: Hu
Applications: WB, ICC
NBP1-90078
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00006464-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, PLA
NBP1-32945
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-04413
Species: Hu
Applications: WB

Publications for GAB2 Lysate (NBP2-04413) (0)

There are no publications for GAB2 Lysate (NBP2-04413).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GAB2 Lysate (NBP2-04413) (0)

There are no reviews for GAB2 Lysate (NBP2-04413). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GAB2 Lysate (NBP2-04413) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GAB2 Products

Bioinformatics Tool for GAB2 Lysate (NBP2-04413)

Discover related pathways, diseases and genes to GAB2 Lysate (NBP2-04413). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GAB2 Lysate (NBP2-04413)

Discover more about diseases related to GAB2 Lysate (NBP2-04413).
 

Pathways for GAB2 Lysate (NBP2-04413)

View related products by pathway.

PTMs for GAB2 Lysate (NBP2-04413)

Learn more about PTMs related to GAB2 Lysate (NBP2-04413).
 

Research Areas for GAB2 Lysate (NBP2-04413)

Find related products by research area.

Blogs on GAB2.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

Customers Who Bought This Also Bought

IL-3 [Unconjugated]
203-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GAB2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GAB2
Entrez