G6PC3 Knockout 293T Cell Lysate


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Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

G6PC3 Knockout 293T Cell Lysate Summary

Knockout achieved by using CRISPR/Cas9,1 bp deletion in exon 1


Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Knockout 293T Cell
Life Stage


Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for G6PC3 Knockout 293T Cell Lysate

  • EC
  • G6Pase 3
  • G-6-Pase 3
  • G6Pase-beta
  • glucose 6 phosphatase, catalytic, 3
  • glucose-6-phosphatase 3
  • Glucose-6-phosphatase beta
  • glucose-6-phosphatase catalytic subunit 3
  • Ubiquitous glucose-6-phosphatase catalytic subunit-related protein
  • ubiquitously expressed G6Pase catalytic subunit-related protein


This gene encodes the catalytic subunit of glucose-6-phosphatase (G6Pase). G6Pase is located in the endoplasmic reticulum (ER) and catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the last step of the gluconeogenic and glycogenolytic pathways. Mutations in this gene result in autosomal recessive severe congenital neutropenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Gene Symbol G6PC3