G protein alpha inhibitor 1 Antibody

Western Blot: G protein alpha inhibitor 1 Antibody [NBP2-16558] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-16558] - human breast cancer, using G protein alpha Inhibitor 1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Discontinued

G protein alpha inhibitor 1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human G protein alpha Inhibitor 1. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Rat (98%), Porcine (98%), Bovine (98%), Guinea Pig (97%), Zebrafish (97%), Chicken (97%), Xenopus (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNAI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (97%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for G protein alpha inhibitor 1 Antibody

• Adenylate cyclase-inhibiting G alpha protein
• Gi
• Gi1 protein alpha subunit
• guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 1
• guanine nucleotide-binding protein G(i) subunit alpha-1

Background

Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for G protein alpha inhibitor 1 Antibody (NBP2-16558)(2)

Publications using NBP2-16558

• Hester A, Salzmann B, Rahmeh M, et al. EP3 receptor antagonist L798,106 reduces proliferation and migration of SK-BR-3 breast cancer cells. Onco Targets Ther. 2019-07-29 [PMID: 31534346] (WB, Human)
• Ye Y, Vattai A, Ditsch N et al. Prostaglandin E2 receptor 3 (EP3) signaling is induced in placentas with unexplained recurrent pregnancy losses (uRPL). Endocr Connect 2018-04-26 [PMID: 29700097] (Human)

Bioinformatics

• Gene Symbol: GNAI1