G protein alpha inhibitor 1 Antibody (L5)

Product Discontinued

G protein alpha inhibitor 1 Antibody (L5) Summary

• Immunogen: Recombinant Gialpha-2, purified by the method of Linder (1990).
• Specificity: Specificity confirmed by Western Blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GNAI1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Application Notes: Western Blot: with detection by 125I-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

Reactivity Notes

Bovine, Guinea Pig, Human, Mouse, Rat
Gialpha-2. Strong reactivity with bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites with 20mM Phosphate (pH 7.6) and 0.25M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Notes

Relevant Entrez Gene Numbers: NM_002070.2

Alternate Names for G protein alpha inhibitor 1 Antibody (L5)

• Adenylate cyclase-inhibiting G alpha protein
• Gi
• Gi1 protein alpha subunit
• guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 1
• guanine nucleotide-binding protein G(i) subunit alpha-1

Background

Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNAI1
• Uniprot:
  • P04899(Human)