Furin Antibody (222712) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clone: 222712
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Furin Antibody (222712) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Furin
  Asp108-Glu715
  Accession # NP_002560
• Specificity: Detects human Furin in ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Furin Antibody (222712) [Alexa Fluor® 405]

• Dibasic-processing enzyme
• EC 3.4.21
• EC 3.4.21.75
• FUR
• FURdibasic processing enzyme
• furin (paired basic amino acid cleaving enzyme)
• Furin
• furin, membrane associated receptor protein
• PACE
• PACEFES upstream region
• paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein)
• Paired basic amino acid residue-cleaving enzyme
• PCSK3
• PCSK3furin
• proprotein convertase subtilisin/kexin type 3
• SPC1

Background

Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine protease (1-3). As a cellular protease, Furin processes a variety of proproteins in secretory pathway compartments by cleaving after Arg-Xaa-Lys/Arg-Arg-like motifs, which usually reside at the end of the pro regions of these proproteins. Examples of the proprotein substrates are growth factors and receptors, extracellular matrix proteins, and other proteases. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. It is synthesized as a 794 amino acid type I transmembrane protein precursor with a signal peptide (residues 1-24), a pro region (residues 25-107), which play a crucial role in the folding, activation and transport of Furin, and a mature chain (residues 108-794) (1-3). The mature chain consists of the subtilisin-like catalytic domain, a P domain, which is essential for enzyme activity and the modulation of pH and calcium requirements, and a cytoplasmic domain, which controls the localization and sorting of Furin in the trans-Golgi network/endosomal system. The purified recombinant human Furin (residues 108-715) corresponds to the mature enzyme terminated before the transmembrane domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.