Fucosyltransferase 3/FUT3 Antibody [Alexa Fluor® 700]

• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

Fucosyltransferase 3/FUT3 Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FUT5
  Arg35-Thr374
  Accession # Q11128
• Specificity: Detects human FUT3 and human FUT5 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

FUT5 ( alpha -1, 3-fucosyltransferase 5; also Fuc-TV) is a 43 kDa member of the glycosyltransferase 10 family. It is responsible for the terminal step in the synthesis of Le^x, sialyl‑Le^x, and Le^a antigens. This is accomplished by the transfer of fucose to preexisting LacNAc or sialyl-LacNAc structures. Human FUT5 is 374 amino acids (aa) in length. It is a type II transmembrane Golgi glycoprotein with a C-terminal lumenal domain (aa’s 35-374). The catalytic domain spans aa’s 77-374, while the acceptor-binding motif includes aa’s 120-126. FUT5 is the product of a single exon gene. Thus, there are no splice variants. FUT5 only occurs in primates; there is no rodent ortholog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.