Frizzled-6 Antibody (211435) [Janelia Fluor® 646]

• Reactivity: Mu
• Applications: WB
• Clone: 211435
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 646

Frizzled-6 Antibody (211435) [Janelia Fluor® 646] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-6
  His19-Val153 (predicted)
  Accession # Q61089
• Specificity: Detects mouse Frizzled-6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Frizzled-5 or recombinant mouse Frizzled-1, -2, -3, -4, -5, -7, -8, or -9 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FZD6
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Frizzled-6 Antibody (211435) [Janelia Fluor® 646]

• frizzled (Drosophila) homolog 6
• frizzled homolog 6 (Drosophila)
• Frizzled6
• Frizzled-6
• FZ6
• fz-6
• FZD6
• Hfz6
• seven transmembrane helix receptor

Background

FZD6 is a Frizzled Receptor for Wnt signaling proteins involved in patterning during development. Contrary to other Frizzled proteins, FZD6 does not contain a C-terminal PDZ domain-binding motif. FZD6 is widely expressed. The Drosophila homolog activates Dishevelled. FZD6 expression has been reported in brain, colon, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, testis, and thymus. Various tumor cell lines have also been shown to express FZD6. ESTs have been isolated from blood, brain, breast, connective tissue, liver, liver/spleen, lung, placenta, testis, thyroid, and vessel libraries. In addition, several ESTs have been isolated from tumor libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FZD6