Frizzled-4 Antibody

Frizzled‑4 was detected in immersion fixed frozen sections of embryonic mouse neuronal tissue (E11) using 15 µg/mL Goat Anti-Mouse Frizzled‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF194) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Frizzled-4 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
  Phe37-Glu180
  Accession # Q8BLL2
• Specificity: Detects mouse Frizzled-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Frizzled-8 and less than 2% cross-reactivity with rmFrizzled-3 and rmFrizzled-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FZD4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-4 Antibody

• CD344 antigen
• CD344
• EVR1
• exudative vitreoretinopathy 1
• FEVR
• frizzled (Drosophila) homolog 4
• frizzled homolog 4 (Drosophila)
• Frizzled4
• Frizzled-4
• Fz-4
• FZD4
• FZD4S
• FzE4
• GPCR
• hFz4
• MGC34390
• WNT receptor frizzled-4

Background

Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120  amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89%, and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).

1. Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
2. Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
3. Xu, Q. et al. (2004) Cell 116:883.
4. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
5. Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
6. Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
7. Wang, Y. et al. (2001) J. Neurosci. 21:4761.
8. Robitaille, J. et al. (2002) Nat. Genet. 32:326.
9. Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-4 Antibody (AF194)(4)

Publications using AF194

• Dattani, A;Corujo-Simon, E;Radley, A;Heydari, T;Taheriabkenar, Y;Carlisle, F;Lin, S;Liddle, C;Mill, J;Zandstra, PW;Nichols, J;Guo, G; Naive pluripotent stem cell-based models capture FGF-dependent human hypoblast lineage specification Cell stem cell 2024-05-24 [PMID: 38823388] (Immunohistochemistry, Human)
• Shen J, Yang X, Xie B, Chen Y, Swaim M, Hackett SF, Campochiaro PA MicroRNAs regulate ocular neovascularization. Mol. Ther., 2008-05-20;16(7):1208-16. 2008-05-20 [PMID: 18500251] (Western Blot, Mouse)
• Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling. J. Biol. Chem., 2008-01-30;283(14):9359-68. 2008-01-30 [PMID: 18234671] (ELISA Development, Mouse)
• Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2. Mol. Biol. Cell, 2006-10-11;17(12):5163-72. 2006-10-11 [PMID: 17035633] (IHC, Mouse)

Bioinformatics

• Gene Symbol: FZD4
• Uniprot:
  • Q8BLL2()