Frizzled-3 Antibody (169310) [Unconjugated]

Frizzled‑3 was detected in immersion fixed paraffin-embedded sections of mouse cortex using Rat Anti-Human/Mouse Frizzled‑3 Monoclonal Antibody (Catalog # MAB1001) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005) . Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Frizzled‑3 was detected in immersion fixed paraffin-embedded sections of human cortex using Rat Anti-Human/Mouse Frizzled‑3 Monoclonal Antibody (Catalog # MAB1001) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005) . Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: IHC
• Clone: 169310
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Frizzled-3 Antibody (169310) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
  Met1-Val157
  Accession # Q61086
• Specificity: Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FZD3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-3 Antibody (169310) [Unconjugated]

• frizzled (Drosophila) homolog 3
• frizzled homolog 3 (Drosophila)
• Frizzled3
• Frizzled-3
• Fz-3
• FZD3
• hFz3

Background

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-3 Antibody (MAB1001)(3)

Publications using MAB1001

• Wong , Sze Chue, He , Catherin, Chan , Charles, Chan , Amanda K, Wong , Heong Ti, Cheung , Moon Ton, Luk , Lewis La, Au , Thomas C, Chiu , Man Kin, Ma , Brigette, Chan , Anthony Clinical significance of frizzled homolog 3 protein in colorectal cancer patients. PLoS ONE, 2013-11-08;8(11):e79481. 2013-11-08 [PMID: 24255701] (ICC, Mouse)
• Li L, Zepeda-Orozco D, Patel V Aberrant planar cell polarity induced by urinary tract obstruction. Am. J. Physiol. Renal Physiol., 2009-09-30;297(6):F1526-33. 2009-09-30 [PMID: 19794107] (IHC-Fr, Western Blot, Mouse)
• Baksh D, Boland GM, Tuan RS Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. J. Cell. Biochem., 2007-08-01;101(5):1109-24. 2007-08-01 [PMID: 17546602] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: FZD3
• Uniprot:
  • Q61086()