Frequenin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Frequenin Antibody [NBP1-78006] - Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Grosse et al., (2000) J. Neurosci. 20: 1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippocampal cell cultures after 19 d in vitro. Numerous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.

• Reactivity: Mu, Hu, Rt, Bv, Ch, Pm-Or, Xp
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Frequenin Antibody - BSA Free Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Frequenin (recombinant) coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications) (Uniprot: Q8BNY6)
• Predicted Species: Human (100%), Rat (100%), Chicken (100%), Xenopus (99%), Bovine (99%), Orangutan (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:2500
  • Western Blot 1:500-1:2000
• Application Notes: This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:5000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Anti-Frequenin has also been tested for use in IF using Hippocampal neurons of 17 day old NMRI mice.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Frequenin Antibody - BSA Free

• DKFZp761L1223
• FREQFLUP
• frequenin (Drosophila) homolog
• frequenin homolog (Drosophila)
• Frequenin homolog
• Frequenin-like protein
• Frequenin-like ubiquitous protein
• NCS-1
• neuronal calcium sensor 1

Background

Frequenin is a Ca2+ binding protein implicated in the regulation of neurotransmitter release at the neuromuscular junction. Frequenin amino acid sequences from different species appear closely related. In mouse a single 4.2 kbp mRNA is produced yielding a protein of 22 kDa when assayed by SDS-PAGE under reducing conditions. Frequenin may colocalize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. In Drosophila frequenin is related to recoverin and visinin and functions like Ca2+-sensitive guanylyl cyclase activator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCS1
• Uniprot:
  • Q8BNY6(Mouse)