FOXO3 Antibody (CL13589) - Azide and BSA Free

Staining of human anaplastic oligodendroglioma shows strong nuclear positivity in neurons.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human gastrointestinal shows weak to moderate nuclear positivity in glandular cells.

Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Analysis in human cell line SH-SY5Y.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL13589
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FOXO3 Antibody (CL13589) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse FOXO3 Antibody (CL13589) - Azide and BSA Free (NBP3-24564) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of O43524, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXO3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for FOXO3 Antibody (CL13589) - Azide and BSA Free

• AF6q21 protein
• AF6q21
• DKFZp781A0677
• FKHRL1
• FKHRL1MGC31925
• FKHRL1P2
• forkhead box O3
• forkhead box protein O3
• forkhead homolog (rhabdomyosarcoma) like 1
• Forkhead in rhabdomyosarcoma-like 1
• forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• FOXO2
• FoxO3
• FOXO3A
• FOXO3Aforkhead box O3A
• MGC12739

Background

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXO3