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FOXO1A/3/4 Antibody (4E7) [DyLight 755]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
4E7
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-41196AF405
Alexa Fluor 488
NBP1-41196AF488
Alexa Fluor 647
NBP1-41196AF647
Alexa Fluor 700
NBP1-41196AF700
Biotin
NBP1-41196B
DyLight 350
NBP1-41196UV
DyLight 405
NBP1-41196V
DyLight 488
NBP1-41196G
DyLight 550
NBP1-41196R
DyLight 650
NBP1-41196C
DyLight 680
NBP1-41196FR
FITC
NBP1-41196F
HRP
NBP1-41196H
Janelia Fluor 549
NBP1-41196JF549
Janelia Fluor 646
NBP1-41196JF646
Unconjugated
NBP1-41196
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FOXO1A/3/4 Antibody (4E7) [DyLight 755] Summary

Immunogen
Amino acids 242-655 of FKHR expressed in E. coli.
Specificity
This antibody will recognize human FKHR.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXO1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



0

Alternate Names for FOXO1A/3/4 Antibody (4E7) [DyLight 755]

  • FKH1
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot: FOXO3 Antibody [NBP2-16521] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: FOXO3 Antibody [NBP2-16521] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXO3A protein stained by FOXO3A antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: FOXO3A Antibody [NBP2-16521] - Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using antibody at 1:500 dilution.
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Insulin Antibody
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Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]
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Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293 using anti-PTK2B antibody. Corresponding PTK2B RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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PI 3-Kinase p110
gamma Antibody
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Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Paraffin-embedded Human Breast Cancer
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Whole cell extract (30ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:1000.
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Applications: WB, IHC, IHC-P
PIK3CA Antibody
(OTI6D9)
NBP2-46403
Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - A. 30 ug PC-12 whole cell lysate/extract 5 % SDS-PAGE PI3K p110 beta antibody [N3C2], Internal dilution: 1:1000
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
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PI 3-Kinase p110
delta Antibody
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Applications: WB, ICC/IF, IHC, IHC-P
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(274212) [Unconjugate
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(OTI1A4)
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Immunohistochemistry: FOXO4 Antibody (1A4) [NBP2-45712] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Sirtuin 1/SIRT1
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IGF-I
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1 μg/lane of Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.
(2 Reviews)
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TOR/mTOR [p
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Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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Pax7 Antibody
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Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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p27/Kip1 [p
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Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291‑G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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PTEN Antibody
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Western blot shows lysates of mouse and rat brain tissue, A431 human epithelial carcinoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human PTEN (5 ng) was included. A specific band for PTEN was detected at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
PTEN was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTEN at approximately 60 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Review)
(5 Publications)
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Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Bim Antibody
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Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Bim Antibody [NBP2-53088] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human endometrial cancer xenograft using Bim antibody at 1:50 dilution. The signal was detected using HRP labeled secondary antibody and DAB staining method which followed counter-staining with hematoxylin. The antibody generated a specific cytoplasmic staining with punctate appearance, signifying Bim/Bcl2L11 presence on mitochondria.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Insulin Receptor
beta Antibody
NBP2-12793
Immunoprecipitation: Insulin Receptor Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
(1 Review)
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Applications: WB, IP
FOXO1A/3/4
Antibody (4E7) [DyLig
NBP1-41196IR
FOXO1A/3/4 Antibody (4E7) [DyLight 755]
Species: Hu
Applications: WB, IHC

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FOXO1A/3/4 NBP1-41196IR

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Bioinformatics

Gene Symbol FOXO1