FoxO1/FKHR Antibody (597554) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 597554
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

FoxO1/FKHR Antibody (597554) [Alexa Fluor® 532] Summary

• Immunogen: E. coli- derived recombinant human FoxO1/FKHR
  Ala353-Gly655
  Accession # Q12778
• Specificity: Detects human FoxO1/FKHR in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxA1 (aa 253-472), B1 (aa 191-324), C1 (aa 208-322), D3 (aa 1-140), F1 (aa 129-354), G1 (aa 283-489), J1 (aa 301-421), J3 (aa 375-552), K1 (aa 493-670
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxO1/FKHR Antibody (597554) [Alexa Fluor® 532]

• FKH1
• FKHR
• FKHRforkhead box protein O1
• forkhead box O1
• Forkhead box protein O1A
• forkhead homolog in rhabdomyosarcoma
• Forkhead in rhabdomyosarcoma
• FoxO1
• FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

FoxO1, also called FKHR (forkhead in ­rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity. A 60 kDa form, cleaved at R537, has been found in androgen-treated prostate cancer cells. Over aa 353-655, human FoxO1 shares 93% aa identity with mouse and rat FoxO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.