FoxM1 Knockout 293T Cell Lysate

Product Discontinued

FoxM1 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-28 bp deletion in exon2 and -25 bp deletion in exon2 and -8 bp deletion in exon2
• Gene: FOXM1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for FoxM1 Knockout 293T Cell Lysate

• FKHL16
• FKHL16trident
• forkhead box M1
• forkhead box protein M1
• Forkhead, drosophila, homolog-like 16
• forkhead-like 16
• Forkhead-related protein FKHL16
• FoxM1
• Hepatocyte nuclear factor 3 forkhead homolog 11
• HFH-11
• HFH11FOXM1B
• HFH-11M-phase phosphoprotein 2
• HNF-3
• HNF-3/fork-head homolog 11
• INS-1
• MPHOSPH2
• MPP-2
• MPP2MPM-2 reactive phosphoprotein 2
• PIG29
• TGT3
• Transcription factor Trident
• Trident
• WIN
• Winged-helix factor from INS-1 cells

Background

The protein encoded by this gene is a transcriptional activator involved in cell proliferation. The encoded protein is phosphorylated in M phase and regulates the expression of several cell cycle genes, such as cyclin B1 and cyclin D1. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: FOXM1