FOXB1 Antibody

Western Blot: FOXB1 Antibody [NB100-1267] - Staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour.

• Reactivity: Mu, Hu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FOXB1 Antibody Summary

• Description: Novus Biologicals Goat FOXB1 Antibody (NB100-1267) is a polyclonal antibody validated for use in WB and ELISA. Anti-FOXB1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TSPASALHSVAVH corresponding to C-Terminus according to NP_036314.2.
• Predicted Species: Human (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot
• Application Notes: ICC: An anonymous customer has reported nuclear staining at lumbar levels of the mouse Spinal Cord at embryonic day 12.5. IF successful usage for mouse brain at embryonic day 13.5. WB: Preliminary experiments gave no signal but low background in human brain extracts at up to 1 ug/ml.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FOXB1 Antibody

• FKH5HFKH-5
• forkhead box B1
• forkhead box protein B1
• Transcription factor FKH-5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOXB1 Antibody (NB100-1267)(2)

Publications using NB100-1267

• Wataya T, Ando S, Muguruma K, Ikeda H, Watanabe K, Eiraku M, Kawada M, Takahashi J, Hashimoto N, Sasai Y. Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation. roc Natl Acad Sci U S A;105(33):11796-801. 2008-08-19 [PMID: 18697938]
• Kloetzli JM, Fontaine-Glover IA, Brown ER et al. The winged helix gene, Foxb1, controls development of mammary glands and regions of the CNS that regulate the milk-ejection reflex. Genesis 2001-02-01 [PMID: 11170346]

Bioinformatics

• Gene Symbol: FOXB1
• Entrez:
  • 27023(Human)
  • 64290(Mouse)
• Uniprot:
  • NP_036314.(Human)