FOLR3 Antibody (553701) [Unconjugated]

Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FOLR3 Monoclonal Antibody (Catalog # MAB5319) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for FOLR3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clone: 553701
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FOLR3 Antibody (553701) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FOLR3 long isoform
  Ala22-Ser245
  Accession # NP_000795
• Specificity: Detects human FOLR3 in direct ELISAs and Western blots. In direct ELISAs, 10% cross-reactivity with recombinant human (rh) FOLR2 is observed and no cross-reactivity with rhFOLR4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOLR3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR3 Antibody (553701) [Unconjugated]

• folate receptor 3 (gamma)
• Folate receptor 3
• folate receptor gamma
• FOLR3
• FR-G
• FR-gamma
• gamma-hFR

Background

Folate receptor 3 (FOLR3), also known as folate receptor gamma , is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1). Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence (2, 3). A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein (2). Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa (2, 4). FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas (2, 3). FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas (5). Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates (3).

1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
2. Shen, F. et al. (1994) Biochemistry 33:1209.
3. Shen, F. et al. (1995) Biochemistry 34:5660.
4. Wang, H. et al. (1998) Nucl. Acids Res. 26:2132.
5. Yuan, Y. et al. (2009) Hum. Pathol. May 18 epub.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOLR3
• Uniprot:
  • NP_000795()