Flt-3 Ligand Antibody (40416) [Unconjugated]

Recombinant Human Flt‑3 Ligand (Catalog # 308-FK) stimulates proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt‑3 Ligand (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt‑3 Ligand Monoclonal Antibody (Catalog # MAB608). The ND50 is typically 0.02‑0.06 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 40416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Flt-3 Ligand Antibody (40416) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand
  Thr27-Pro185
  Accession # AAA17999
• Specificity: Detects human Flt-3 Ligand in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT3LG
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.02-0.06 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human Flt-3 Ligand Biotinylated Antibody (Catalog number BAF308)
  Standard: Recombinant Human Flt-3 Ligand (Catalog number 308-FKN)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Flt-3 Ligand Antibody (40416) [Unconjugated]

• FL
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flt-3 Ligand Antibody (MAB608)(2)

Publications using MAB608

• Anandasabapathy N, Breton G, Hurley A, Caskey M, Trumpfheller C, Sarma P, Pring J, Pack M, Buckley N, Matei I, Lyden D, Green J, Hawthorne T, Marsh H, Yellin M, Davis T, Keler T, Schlesinger S Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers. Bone Marrow Transplant, 2015;50(7):924-30. 2015 [PMID: 25915810] (ELISA Development (Capture), Human)
• Briard D, Brouty-Boye D, Azzarone B, Jasmin C Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol., 2002;168(9):4326-32. 2002 [PMID: 11970974] (Neutralization, Human)

Bioinformatics

• Gene Symbol: FLT3LG
• Uniprot:
  • AAA17999()