Flt-3 Ligand Antibody (40406) [Unconjugated]

Recombinant Human Flt‑3 Ligand (Catalog # 308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt‑3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt‑3 Ligand Monoclonal Antibody (Catalog # MAB308). The ND50 is typically 0.02‑0.06 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 40406
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Flt-3 Ligand Antibody (40406) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand
  Thr27-Pro185
  Accession # AAA17999
• Specificity: Detects human Flt-3 Ligand in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse Flt‑3 Ligand.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT3LG
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Neutralization 0.02-0.06 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Flt-3 Ligand Antibody (40406) [Unconjugated]

• FL
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flt-3 Ligand Antibody (MAB308)(3)

Publications using MAB308

• Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015;290(37):22338-51. 2015 [PMID: 26183780] (Neutralization, Mouse)
• Sailaja G, Husain S, Nayak BP, Jabbar AM Long-term maintenance of gp120-specific immune responses by genetic vaccination with the HIV-1 envelope genes linked to the gene encoding Flt-3 ligand. J. Immunol., 2003;170(5):2496-507. 2003 [PMID: 12594275] (ELISA Development, Human)
• Kinasewitz GT, 2016, Chang AC, Peer GT, Hinshaw LB, Taylor FB Peritonitis in the baboon: a primate model which stimulates human sepsis., 2000;13(2):100-9. 2000 [PMID: 10670839] (ELISA Development, Primate - Papio cynocephalus (Yellow Baboon))

Bioinformatics

• Gene Symbol: FLT3LG
• Uniprot:
  • AAA17999()