FKBP51/FKBP5 Antibody (425314)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP51 Monoclonal Antibody (Catalog # MAB4094) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 425314
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

FKBP51/FKBP5 Antibody (425314) Summary

• Immunogen: E. coli-derived recombinant human FKBP51
  Met1-Val457
  Accession # Q13451
• Specificity: Detects endogenous human, mouse and rat FKBP51 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: FKBP5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FKBP51/FKBP5 Antibody (425314)

• 51 kDa FKBP
• 54 kDa progesterone receptor-associated immunophilin
• AIG6,51 kDa FK506-binding protein
• Androgen-regulated protein 6
• Dit1
• FF1 antigen
• FK506 binding protein 5
• FK506-binding protein 5
• FKBP5
• FKBP-5
• FKBP51
• FKBP-51
• FKBP51PPIase FKBP5
• FKBP54
• FKBP54EC 5.2.1.8
• HSP90-binding immunophilin
• MGC111006
• p54
• P54,51 kDa FK506-binding protein 5
• peptidyl-prolyl cis-trans isomerase FKBP5
• peptidylprolyl cis-trans isomerase
• PPIase
• Ptg-10
• rotamase
• T-cell FK506-binding protein

Background

FK506 binding protein (FKBP51) is a 51 kDa peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans- proline residues critical for proper folding of proteins. The macrolide immunosuppressants FK506 and Rapamycin are FKBP51 inhibitors. FKBP51 levels are induced by glucocorticoids, and it associates with HSP90 complexes that are critical for the proper folding of steroid receptors. Single nucleotide polymorphisms in FKBP51 have been associated with major depression and hyper-responsiveness to antidepressants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FKBP51/FKBP5 Antibody (MAB4094)(3)

Publications using MAB4094

• E Navarro-Vi, P de la Cruz, L Contreras, R González, M Negrete, MA Rodríguez-, LM Marín-Góme, JM Álamo-Mart, A Calvo, MA Gómez-Brav, J de la Cruz, J Padillo, J Muntané Molecular Pathways Leading to Induction of Cell Death and Anti-Proliferative Properties by Tacrolimus and mTOR Inhibitors in Liver Cancer Cells Cell. Physiol. Biochem., 2020-05-06;54(3):457-473. 2020-05-06 [PMID: 32369692] (Western Blot, Human)
• AJ Lesiak, K Coffey, JH Cohen, KJ Liang, C Chavkin, JF Neumaier Sequencing the serotonergic neuron translatome reveals a new role for Fkbp5 in stress Mol. Psychiatry, 2020-05-04;0(0):. 2020-05-04 [PMID: 32366949] (IHC, Mouse)
• Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R Neuropsin cleaves EphB2 in the amygdala to control anxiety. Nature, 2011-04-20;473(7347):372-5. 2011-04-20 [PMID: 21508957] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: FKBP5
• Uniprot:
  • Q13451()