Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)

FAP was detected in immersion fixed paraffin-embedded sections of human Melanoma using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm of fibroblasts. Protocol available in COMET™ Panel Builder.

FAP was detected in immersion fixed paraffin-embedded sections of human Colon Cancer using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm of fibroblasts. Protocol available in COMET™ Panel Builder.

FAP was detected in immersion fixed paraffin-embedded sections of human Pancreas using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of pancreatic islet cells. Protocol available in COMET™ Panel Builder.

Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Whole cell lysate (500 ug per IP reaction; 5% of IPloaded) from IMR-90 cells. Antibody: Rabbit anti-FAPrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blottingimmunoprecipitated FAP, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.

Western Blot-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti FAP recombinant monoclonal antibody [BLR150J] used at 1:1000. Secondary: HRP conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Immunohistochemistry-Paraffin-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.

ICC/IF- Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP, mIF
• Clone: BLR150J
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) Summary

• Description: Novus Biologicals Rabbit Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) (NBP3-14728) is a recombinant monoclonal antibody validated for use in Multiplex Immunofluorescence, IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: residues 26-760 (ECD)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FAP
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 to 1:500
  • Immunohistochemistry-Paraffin 1:100 to 1:500
  • Immunoprecipitation 20 µl/1 mg lysate
  • Multiplex Immunofluorescence 1:100
  • Western Blot 1:1,000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAP